Cell lysate in 1% TX100 was purified over an affinity column to isolate a complex with certain enzymatic activity. The purified enzyme complex was separated on a 10-50% continuous sucrose gradient. Shown below are the UV spectra using an absorbance filter at 280 nm or 260 nm.

Which one of the following combinations of molecules would generate the spectra shown above?

1
Protein-Protein
2
Protein-RNA
3
Protein-Lipid
4
Protein-Cholesterol

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