Which of the following will NOT be triggered by the release of acetyl choline in the synapse at the neuromuscular junction during muscle contraction?

1
Generation of an action potential in the muscles
2
Release of Ca2+ from the sarcoplasmic reticulum
3
Binding of ATP molecules to the myosin head
4
Shifting of tropomyosin to expose the myosin binding sites on actin filaments

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