Which of the following best describes the role of the LIN-3 ligand in C. elegans vulva induction?

1
LIN-3 disinhibits the activity of the EGF receptor LET-23, preventing vulval development
2
LIN-3 is produced by the VPCs themselves to promote their own division and differentiation independently of the gonad signals.
3
LIN-3 acts as an inhibitor of the LET-60 Ras protein, indirectly controlling vulval formation by modulating signal transduction.
4
LIN-3, produced by the anchor cells, binds to the EGF receptor LET-23 on VPCs, initiating a signaling cascade essential for vulval development.

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