How does chloramphenicol inhibit bacterial protein synthesis?

1
It binds to the 30S ribosomal subunit and prevents peptide bond formation.
2
It binds to the 50S ribosomal subunit and prevents the elongation of the polypeptide chain.
3
It prevents the initiation of translation by blocking the binding of mRNA to the ribosome.
4
It inhibits the activity of aminoacyl-tRNA synthetase, preventing tRNA loading.

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