Activin-secreting beads were placed on unspecified cells from an early Xenopus embryo. The Activin then diffused from the beads. At high concentrations (~300 molecules/cell), Activin would induce the expression of the

1
Both Xbra and Goosecoid gene
2
Xbra gene 
3
Goosecoid gene
4
Oct 4 gene

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