Topoisomerase activity was measured in terms of change in the linking number of DNA in the presence of Camptothecin (inhibitor of Topoisomerase I) or Etoposide (inhibitor of Topoisomerase II). Which one of the following is the correct expected outcome?

1
In the presence of Camptothecin, Topoisomerase I will lead to change in the linking number by ±2.
2
In the presence of Etoposide, Topoisomerase I will lead to change in the linking number by ±2.
3
In the presence of Camptothecin, Topoisomerase II will lead to change in the linking number by ±2.
4
In the presence of Etoposide, Topoisomerase II will lead to change in the linking number by ±2.

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