Teaching MPPSC Assistant Professor Mock Test Series 2025 Life Sciences Fundamental Processes RNA synthesis and processing
With an intention to identify the genes expressed in an organism at specific stage of development, mRNAs were isolated from the given organism, cDNAs were synthesized, cloned in a suitable vector and sequenced. A few of the cDNA sequences showed no matches with the genomic DNA sequence. Further, it was observed that these sequences were U-rich and found to be in stretches dispersed along the sequence. The following may be possible reasons for appearance of such RNA:
- Splicing
- Alternate splicing
- Trans-splicing
- Guide RNA mediated introduction of Us involving endonuclease, terminal-U transferase and RNA ligase
- Deaminations converting C to U
Which of the following is the most appropriate reason/s?
1
1 and 3
2
4 and 5
3
2 and 4
4
4 only