The sequence of monosaccharides including position and configuration of glycosidic bonds in a glycoprotein is to be determined. Which one of the following methods can be employed?
1
Glycoprotein → removal of oligosaccharides by alkaline hydrolysis → nuclear magnetic resonance analysis of cleaved mixture of oligosaccharides
2
Two dimensional nuclear magnetic resonance spectroscopic analysis of the glycoprotein
3
Glycoprotein → release of oligosaccharides with endoglycosidases followed by purification to separate oligosaccharides → enzymatic hydrolysis of purified oligosaccharides with specific glycosidases → mass spectroscopic analysis of smaller oligosaccharides
4
Glycoprotein → treat with trypsin followed by MALDI analyses of tryptic peptides