Teaching MPPSC Assistant Professor Mock Test Series 2025 Life Sciences Cellular Organization Membrane structure and function
Which of the following statements best describes the methods used for isolating peripheral and integral proteins from cell membranes?
1
Peripheral proteins can be isolated using high concentrations of NaCl, which disrupts ionic and hydrogen bonds, detaching these proteins from the membrane without affecting the lipid bilayer integrity. Integral proteins require the use of detergents to dissolve the lipid bilayer and release the proteins embedded within it.
2
Both peripheral and integral proteins are effectively isolated using only high concentrations of NaCl, as this salt solution can disrupt the lipid bilayer and release all types of proteins from the membrane.
3
Detergents are necessary for isolating peripheral proteins because they help to break the weak interactions between these proteins and the membrane lipids. In contrast, integral proteins can easily be extracted using a simple buffer solution without any additives.
4
High concentrations of NaCl are essential for isolating integral proteins by causing the phospholipid bilayer to dissolve, while peripheral proteins can be extracted by applying low concentrations of detergents to preserve the membrane's structural integrity.