A 2 Kb insert has to be ligated to a 8 Kb plasmid in a ligation mix where we want to keep the vector insert molar ratio as 1: 2. If 1 µg of vector is used, the amount of insert to be used is:

1
2 µg
2
1 µg
3
0.5 µg
4
0.25 µg

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