Teaching MPPSC Assistant Professor Mock Test Series 2025 Life Sciences Biochemistry Enzymes and Enzyme kinetics
The Lineweaver Burk plot is used to graphically determine Km and Vmax for an enzyme that obeys classic Michaelis Menten kinetics. When V is the reaction velocity at substrate concentration S, the Y axis experimental data in the Lineweaver Burk plot are expressed as
1
V
2
1/V
3
S
4
1/S