Erythromycin is an inhibitor of protein synthesis. It acts by:

1
binding to 30S subunit of bacterial ribosome, thus inhibiting binding of aminoacyl-tRNAs.
2
binding to 50S subunit of bacterial ribosome, thus inhibiting translocation.
3
inhibits peptidyl transferase activity of eukaryotic 60S ribosomal subunit.
4
causes premature chain termination by acting as an analog of aminoacyl-tRNA in both prokaryotes and eukaryotes.

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