Teaching MPPSC Assistant Professor Mock Test Series 2025 Life Sciences Methods in Biology Radiolabeling techniques
Fluorescence Recovery After Photobleaching (FRAP) is a widely used technique to study the dynamics of molecular diffusion and mobility within cellular membranes. During a FRAP experiment, a specific area of a cell membrane containing fluorescently labeled lipids is photobleached, and the recovery of fluorescence is monitored over time. What does the rate of fluorescence recovery primarily indicate in a FRAP experiment?
1
The photostability of the fluorescent dye used in the experiment.
2
The diffusion coefficient and mobility of the fluorescently labeled molecules within the membrane.
3
The intensity of fluorescence before photobleaching.
4
The energy efficiency of the photobleaching laser