In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

1
Genetically engineered lymphocytes are not immortal cells.
2
Retroviral vector is introduced into these lymphocytes.
3
Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages
4
More than one of the above
5
None of the above

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