In the Lineweaver–Burk plot of initial rate–1 vs. initial substrate concentration–1 for an enzyme catalyzed reaction, following Michaelis Menten mechanism, the y–intercept is 5000 M–1 s. If the initial enzyme concentration is 1 × 10 9 M, the turnover number is:
1
2.5 × 103
2
1.0 × 104
3
2.5 × 104
4
2.0 × 105