Which of the following changes occur in protein due to denaturation?

A. Solubility of protein increases.

B. Solubility of protein decreases.

C. The peptide bonds of protein are readily available for hydrolysis by proteolytic enzymes. 

D. Viscosity and optical rotation increases.

E. Biological properties such as catalytic, hormonal etc. are lost.

Choose the most appropriate answer from the options given below: