What is the sequence of steps involved in conducting Radio Frequency lesion by using stereotoxic apparatus ?

A. Drill a hole in the skull of the rat using coordinates from the stereotoxic atlas.

B. Pass on radio frequency current through the tip of the insulated electrode to the brain area.

C. Close the hole on the skull of the rat and provide appropriate medical treatment.

D. Fix the head of the rat on the stereotoxic apparatus

E. Push an electrode to the appropriate depth of the brain till it touches the specified brain area.

Choose the correct answer from the options given below: